Syphilis antibody confirmatory test
Positive –syphilis-screening results have to be confirmed by using tests like FTA-ABS to differentiate the immunoglobuline subclasses like IgG or IgM.
FTA-ABS – Fluorescence Treponema-Antibody Absorption test
Treponema (Nichols strain) are immobilised as antigen on slides. Specific FITC conjugated anti human IgG (anti gamma chain) or IgM (anti µ chain) is used to determine the individual immunoglobuline class. For the determination of IgM it has to be separated from IgG.
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